Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions.
نویسندگان
چکیده
A procedure has been developed in which Salmonella can be detected in dried foods and feeds within 50 hr. This includes preenrichment (18 hr), selective enrichment (24 hr), elective enrichment (6 to 8 hr), and serological testing (2 hr). The procedure is as sensitive as the more time-consuming, traditional (plating, biochemical, and serological) procedure which may require at least 4 to 5 days. The new procedure is rapid and accurate in comparison to traditional procedures. Moreover, it is simple and inexpensive to perform. As described, the procedure does not necessitate the isolation of pure cultures, but this step is performed easily if desired.
منابع مشابه
Evaluation of an automated fluorescent antibody procedure for detection of Salmonella in foods and feeds.
A prototype automated system using fluorescent antibody (FA) was evaluated for rapid detection of salmonellae in foods. Samples were enriched in selenite cystine and tetrathionate broths. After incubation, both were transferred into fresh selenite cystine for a 4-h "post-enrichment" to dilute possible background fluorescence from product. These cultures were then analyzed automatically, and res...
متن کاملAccelerated immunofluorescence procedure for the detection of Salmonella in foods and animal by-products.
An accelerated, direct immunofluorescent-antibody procedure was developed for the detection of Salmonella in food products. This method includes pre-enrichment and selective enrichment but eliminates many of the washing and smear treatments present in existing methods. Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, bi...
متن کاملDetection of Salmonella spp contamination of carcasses slaughtered in poultry abattoir in Mashhad, Iran
A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad. The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping. PCR amplification of invA gene as a specific method for detection of Salmonella w...
متن کاملRapid detection of Salmonella in foods using a combination of SPRINTTM, MSRVTM and Salmonella Latex TestTM
A new procedure for rapid detection of Salmonella in foods, based on the combination of SPRINTTM, MSRVTM and Salmonella Latex TestTM, was evaluated. SPRINTTM is a system to reduce the preenrichment and selective enrichment steps to 24 hours. MSRVTM is a semi-solid selective media for detection of motile Salmonella. Salmonella Latex TestTM is a rapid latex agglutination test for Salmonella. Usin...
متن کاملSalmonella isolated from feeds and feed ingredients during the period 1982-1988: animal and public health implications.
The prevalence of Salmonella in southern Africa in farm feeds and by-products of animal origin during 1982-1988 was determined. Salmonella occurred in 5.18% of the farm feed samples and in 9.54% of the by-product samples. Different serovars were isolated, some only once. The findings underestimate the true prevalence of Salmonella in farm feeds and by-products, and is representative of only the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied microbiology
دوره 17 4 شماره
صفحات -
تاریخ انتشار 1969